Introgression of Seedling Plant Resistance to Leaf Rust from Agropyron cristatum into Wheat by Induced Homoeologous Recombination

Agropyron cristatum (P genome) is a Triticeae species from the wheat tertiary gene pool which has economic importance as forage and also displays traits beneficial to wheat. Resistance leaf rust was previously mapped short arm of chromosome 1P (1PS) in A. by development compensating Robertsonian translocation involving 1PS long 1B (1BL). In this study, engineered using ph1b mutation induce 1BS/1PS homoeologous recombination obtain new translocations with shortened fragments 1PS. Two different alien fragment sizes were identified genomic situ hybridization, 1BS- 1PS-specific molecular markers gene-specific for glutenin, Glu-B3 gliadin Gli-B1 seed storage protein. One (called type 1) replaces proximal segment chromatin, other 2) distal introduces protein loci. Six specific EST-STS amplified PCR products recombinant 2 line. analysis showed that highly resistant virulent race pathogen. The wheat–A. obtained yield material seedling plant resistance